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It is because adenine launched to the new DNA strand is unmethylated. Re-methylation occurs inside of two to four seconds, during which period replication errors in The brand new strand are fixed. Methylation, or its absence, would be the marker which allows the maintenance equipment in the cell to

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Pyrosequencing of bisulfite addressed DNA. This is actually the sequencing of the amplicon created by a standard forward primer but a biotinylated reverse primer to PCR the gene of alternative. The Pyrosequencer then analyses the sample by denaturing the DNA and including 1 nucleotide at a time to t
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